The University of Arizona
Please note that this event has ended!

Computational estimates of mechanical constraints on cell migration through the extracellular matrix

Modeling, Computation, Nonlinearity, Randomness and Waves Seminar

Computational estimates of mechanical constraints on cell migration through the extracellular matrix
Series: Modeling, Computation, Nonlinearity, Randomness and Waves Seminar
Location: Online
Presenter: Wanda Strychalski, Department of Applied Mathematics, Case Western Reserve University

Cell migration through a three-dimensional (3D) extracellular matrix (ECM) underlies important physiological phenomena and is based on a variety of mechanical strategies depending on the cell type and the properties of the ECM. Using computational simulations, we investigate two such migration mechanisms: 'push-pull' (forming a finger-like protrusion, adhering to an ECM node, and pulling the cell body forward) and 'rear-squeezing' (pushing the cell body through the ECM by contracting the cell cortex and ECM at the cell rear). We present a computational model that accounts for both elastic deformation and forces of the ECM, an active cell cortex and nucleus, and for hydrodynamic forces and flow of the extracellular fluid, cytoplasm, and nucleoplasm. The model is formulated using the method of regularized Stokeslets to simulate fluid-structure interactions. We find that relations between three mechanical parameters, the cortex's contractile force, nuclear elasticity, and ECM rigidity, determine the effectiveness of cell migration through the dense ECM. The cell can migrate persistently even if its cortical contraction cannot deform a near-rigid ECM, but then the contraction of the cortex has to be able to sufficiently deform the nucleus. The cell can also migrate even if it fails to deform a stiff nucleus, but then it has to be able to sufficiently deform the ECM. Simulations show the rear-squeezing mechanism of motility results in more robust migration with larger cell displacements than those with the push-pull mechanism over a range of parameter values. Additionally, results show that the rear-squeezing mechanism is aided by hydrodynamics through a pressure gradient.

Zoom:  https://arizona.zoom.us/j/94534134312
Password:  “arizona” (all lower case)